add pieces in to plasmid maps snapgene viewer

Data are shown for each cell type grown in the presence or absence of 0.0012% (w/v) arabinose.

OD600 was measured every hour from 0 to 8 h cultivation time and data were plotted using the Growthcurve package in R software. a Growth analysis of double integrants ΔarsB::EGFP-hDHFRΔlacZ::RJ1 (iEGFP-hDHFR_iRJ1), ΔarsB::RzIEGFP-hDHFRΔlacZ::RJ1 (iRzI_iRJ1), ΔarsB::RzIIEGFP-hDHFRΔlacZ::RJ1 (iRzII_iRJ1), ΔarsB::glmSEGFP-hDHFRΔlacZ::RJ1 (iglmS_iRJ1), and ΔarsB::M9EGFP-hDHFRΔlacZ::RJ1 (iM9_iRJ1). Growth analysis and fluorescence intensity of iRJ1 double integrants. P-values from single value two-tailed t-tests comparing group mean to 1 of pEGFP-hDHFR_iRJ1, pRzI_iRJ1, and pRzII_iRJ1 are 0.005381, 0.04022, and 0.03996, respectively Dots indicate relative fluorescence data from individual experiments, and median values are indicated by the bold black line. Box plots show relative fluorescence data distribution. c Fluorescence intensity of pEGFP-hDHFR_iRJ1, pRzI_iRJ1, and pRzII_iRJ1 integrants cultured in 0.0012% (w/v) arabinose relative to untreated control. P-values from two sample t-tests for pEGFP-hDHFR_iRJ1, pRzI_iRJ1, and pRzII_iRJ1 integrants comparing growth in the presence or absence of 0.0012% (w/v) arabinose are 0.00427, 0.00523, and 0.00373, respectively. Points represent the mean of 5 experiments and error bars represent 95% confidence intervals. b Growth analysis of iRJ1 integrants harboring reporter plasmids, pEGFP-hDHFR (pEGFP-hDHFR_iRJ1), pRzIEGFP-hDHFR (pRzI_iRJ1), and pRzIIEGFP-hDHFR (pRzII_iRJ1). Lane M indicates KaledioscopeTM prestained protein ladder (Bio-Rad). The expected size of RNaseJ1-6xHis is 63 kDa, as shown in the arrow. Total protein-stained membrane is shown in the left panel and immunodetection of RNaseJ1-6xHis using Anti-6X His IgG, CF™680 (Sigma-Aldrich, Merck KGaA, Germany) is shown in the right panel. coli BL21(DE3) with integration of pBAD33-containing control DNA fragment (iBAD33, lane 1–3) and RNaseJ1-expressing gene cassette (iRJ1, lane 4–6) cultured in 0, 0.0012, and 0.0037% (w/v) arabinose as shown by the wedge above the lanes. a Western immunoblot analysis to detect RNaseJ1-6xHis protein expressed in E. Phenotypic analysis of iRJ1 integrants harboring reporter plasmids. Points represent mean of 2–4 experiments and error bars represent 95% confidence intervals OD600 was measured every hour from 0 to 8 h cultivation time in cultures grown in HDAG medium with no arabinose and data were plotted using the Growthcurve package in R software. coli BL21(DE3) co-transformants of pRSETC/ pBAD33-rnjA-6xHis (pRSETC_pRJ1), pEGFP-hDHFR/ pBAD33-rnjA-6xHis (pEGFP-hDHFR_pRJ1), pRzIEGFP-hDHFR/ pBAD33-rnjA-6xHis (pRzI_pRJ1), pRzIIEGFP-hDHFR/ pBAD33-rnjA-6xHis (pRzII_pRJ1). The expected size of RNaseJ1-6xHis is 63 kDa, as shown by the arrow. coli DH5α harboring pBAD33 (pBAD33, lane 1–3) and pBAD33-rnjA-6xHis (pRJ1, lane 4–6) cultured in 0, 0.0012, and 0.0037% (w/v) arabinose as shown by the wedge above the lanes. a Western immunoblot analysis to detect RNaseJ1-6xHis in E. Phenotypic analysis of plasmid transformants.

Add pieces in to plasmid maps snapgene viewer